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Solid Phase Inc pcr-direct sequence method
Pcr Direct Sequence Method, supplied by Solid Phase Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr-direct sequence method/product/Solid Phase Inc
Average 90 stars, based on 1 article reviews
pcr-direct sequence method - by Bioz Stars, 2026-06
90/100 stars

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Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

Journal: BMC Genomics

Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

doi: 10.1186/s12864-022-08386-y

Figure Lengend Snippet: Comparative analysis of aligned sequencing reads on the human BCL2L12 gene, derived from the presented 5′ RACE-seq approach and publicly available Direct RNA sequencing datasets. A Visualization of the aligned sequencing reads derived from the presented 5′ RACE-seq approach. B Visualization of the aligned sequencing reads derived from publicly available Direct RNA sequencing datasets deposited on the Sequence Read Archive database (SRA). C Indicative raw nanopore sequencing reads from the generated FASTQ file of the presented 5′ RACE-seq approach, highlighting the incorporation of the drTSO specifically to the 5′ end of each target mRNA transcript

Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

Techniques: Sequencing, Derivative Assay, RNA Sequencing, Nanopore Sequencing, Generated

Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

Journal: BMC Genomics

Article Title: A versatile 5′ RACE-Seq methodology for the accurate identification of the 5′ termini of mRNAs

doi: 10.1186/s12864-022-08386-y

Figure Lengend Snippet: Template-switching oligonucleotides (TSOs) and 5′ RACE primers used in the present work

Article Snippet: Our results led to the accurate identification of the 5′ UTRs of the BCL2L12 gene, being in accordance with the 5′ ends provided by both direct RNA and PCR-cDNA sequencing method from Oxford Nanopore Technologies.

Techniques: Sequencing, Modification